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Updated: Jun 10, 2026

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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. In an alkaline medium, potassium ferricyanide oxidizes hemoglobin to methemoglobin. Further reaction with potassium cyanide produces cyanmethemoglobin which has a maximum absorbance at 540 nm. Color intensity is proportional to total hemoglobin concentration, which is how the hemoglobin levels are measured.
Hematology
In the hemoglobin methodology using potassium ferricyanide the following measurable reaction occurs:

A) carboxhemoglobin is formed
B) iron remains in the ferrous state
C) acid hematin is formed
D) ferrous iron is oxidized to ferric iron to form methemoglobin


2. To obtain a serum sample for a stat test on a patient receiving anticoagulant therapy, the recommended tube is:
Question options:

A) red/gray or gold
B) yellow/gray or orange
C) light green
D) red


3. A zone of inhibition is the area around an antibiotic-infused paper disk that does not show any bacterial growth. The antibiotic impregnated on the disk will diffuse into the agar in the area surrounding the disk. If the bacteria are sensitive to the antibiotic, they cannot grow near the disk. The size of the zone is proportional to how sensitive the organism is. If the organism is resistant to the antibiotic, it will grow very closely to the disk.
The size of the zone of suppressed growth on a sensitivity plate using sensitivity disks is referred to as the zone of:

A) alpha hemolysis
B) susceptibility
C) beta hemolysis
D) gamma hemolysis
E) inhibition


4. Patients with antibody to the following antigen are immune to Hepatitis B:

A) Core antigen
B) antigen
C) Delta antigen
D) Surface antigen


5. hs-CRP is a recent marker of chronic inflammation. New measurements of CRP in lower levels (hs-CRP) are now measured to monitor risk of cardiovascular disease.
Select the statement that best describes hs-CRP?

A) hs-CRP is a measurement of acute inflammation and is used to monitor these conditions
B) hs-CRP is an anti-inflammatory adipokine synthesized by adipocytes
C) hs-CRP is a marker of chronic inflammation and measured to predict the risk of cardiovascular disease
D) hs-CRP is decreased in inflammatory conditions and measured to predict a return of inflammation


Solutions:

Question # 1
Answer: D
Question # 2
Answer: B
Question # 3
Answer: E
Question # 4
Answer: D
Question # 5
Answer: C

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